Macvector attb recombination6/5/2023 ![]() ![]() At the end of the activity, students should be able to depict and explain the overall sequence of events that occur in attB/attP recombination, apply this knowledge to predict what happens with a novel DNA molecule when using this technique, and explain how this reaction can be used to clone a target DNA molecule. The HK and C31 tools were developed by placing the attB sites from each system into the signal peptide cleavage site coding sequence of bla. ![]() Students then explore an example of how the recombination reaction has been exploited to accomplish a cloning reaction. Integration of bacteriophage into the Escherichia coli genome occurs by recombination between the phage attachment (attP) and the bacterial attachment (attB) regions. First BRC0566 antIs31 unc-119 (ed9) was outcrossed to N2 to remove unc-119 ( ed9) to yield BRC0664. In this study, we show that the mv4 integ. The chromosomal inversion was generated as follows. Recombination is catalyzed by the phage-encoded integrase and occurs between the phage attP site and the bacterial attB site. Students first explore a simplified mechanism of the process to familiarize themselves with the recombination reaction, followed by an application of these ideas using a novel DNA molecule. The attB site for Joe integrase was occupied in several Streptomyces genomes, including that of S. recombination has a strong directional bias in response to environmental conditions. Recombination was used to place the attB and attP insertions onto the same chromosome to generate BRC0671 ( antSi50 antSi51 ). The following activity is designed for undergraduate biology students who will use this technique in a lab setting and introduces them to how this method works, and its utility in molecular cloning. AttB/attP-mediated recombination is a technique used in molecular biology labs to clone a target DNA molecule. ![]()
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